Never place tissue in formalin or
Do not collect tissue using
Do not place any tissue in
heparinized containers, or tubes with heparin solution
Do not use heparin when collecting
CT- or ultrasound-guided aspirates, core biopsies, FNA specimens
or bone marrow.
Do not put core biopsies in OCT
Tissue blocks cannot be used to
Except for peripheral blood, all
specimens should be kept cold (either on 'wet' or dry ice) to
prevent RNA degradation.
If transporting a specimen on 'wet'
ice, cover fresh tissue with normal saline to reduce RNA
Freshly excised lymph nodes (LN)
are the preferred tissue. Tissue should be immediately
placed in an appropriate container (tight screw top) of normal
saline and shipped on 'wet' ice.
Amount of LN tissue required to
minimum dimension of ~ 0.3 cm
2-3 smaller specimens are
better than one large sample, but avoid creating 'diced'
Excisional lymph nodes should
be less than or equal to 1.0 gram.
Collect core biopsies using an 1-14
gauge needle to yield a core biopsy ~0.2 cm in diameter by at
least 1 cm in length.
Core biopsy snap freezing must
occur immediately to freeze the tissue solid to prevent RNA
Immediately place in a
container and placing on dry ice until frozen solid (~ 10
Immediately place in liquid
nitrogen for ~ 20 seconds or, in ultra low temperature freezer
(-80 C) for ~ 10 minutes.
Fine Needle aspirates:
provide 2-3 aspirates (.1 - .2
cc each) in separate containers.
number aspirates in order of
bloody aspirates are unlikely
to yield adequate RNA.
Bone marrow and other core
Peripheral blood must display an
absolute lymphocyte count of 5 x 106 cells/mL by manual
differential. 7-10 mL of blood is needed, collected in EDTA tubes